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polyclonal goat anti human tnfsf15  (R&D Systems)
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Gene ontology of 25 selected genes from microarray analyses.
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polyclonal rabbit antiphospho lats2 ser872  (Santa Cruz Biotechnology)
96
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Figure 2. The effect of renal proximal tubule-specific <t>Lats2</t> ablation on kidney histopathology after I/R injury. (A) Schematic representation of Lats2-CKO mice established by crossing Lats2flox/flox with γGgt1-Cre+/+ mice. (B) Identification of genotype Lats2flox/flox; γGgt1-Cre+/+ mice by PCR-based genotyping. (C) Lats2-Ctrl and Lats2-CKO mice were subjected to 40 min warm U-IRI. (D) Serum KIM-1 levels of mice were determined by ELISA in sham and U-IRI mice 2 days after U-IRI (sham n = 4, I/R 2d n = 5). (E) Western blotting analysis of LATS2, P-LATS2 <t>(Ser872)</t> in sham and IRI 14d mice and (F) quantified and normalized to GAPDH expression. (G) Representative HE staining of kidney in Lats2-Ctrl and Lats2-CKO mice subjected to U-IRI. Scale bars, 100 µm. (H) Semi-quantification of tubulointerstitial damage (sham n = 4, I/R 14d n = 5). Data expressed as means ± SEM. Two-way ANOVA followed by Tukey’s multiple comparisons post hoc test. * p < 0.05, ** p < 0.01 *** p < 0.001, **** p < 0.0001 defined as significant. ns, not statistically significant.
Polyclonal Rabbit Antiphospho Lats2 Ser872, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat polyclonal ltα capture antibody  (R&D Systems)
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Figure 2. The effect of renal proximal tubule-specific <t>Lats2</t> ablation on kidney histopathology after I/R injury. (A) Schematic representation of Lats2-CKO mice established by crossing Lats2flox/flox with γGgt1-Cre+/+ mice. (B) Identification of genotype Lats2flox/flox; γGgt1-Cre+/+ mice by PCR-based genotyping. (C) Lats2-Ctrl and Lats2-CKO mice were subjected to 40 min warm U-IRI. (D) Serum KIM-1 levels of mice were determined by ELISA in sham and U-IRI mice 2 days after U-IRI (sham n = 4, I/R 2d n = 5). (E) Western blotting analysis of LATS2, P-LATS2 <t>(Ser872)</t> in sham and IRI 14d mice and (F) quantified and normalized to GAPDH expression. (G) Representative HE staining of kidney in Lats2-Ctrl and Lats2-CKO mice subjected to U-IRI. Scale bars, 100 µm. (H) Semi-quantification of tubulointerstitial damage (sham n = 4, I/R 14d n = 5). Data expressed as means ± SEM. Two-way ANOVA followed by Tukey’s multiple comparisons post hoc test. * p < 0.05, ** p < 0.01 *** p < 0.001, **** p < 0.0001 defined as significant. ns, not statistically significant.
Goat Polyclonal Ltα Capture Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyclonal af7449  (R&D Systems)
94
R&D Systems polyclonal af7449
Figure 2. The effect of renal proximal tubule-specific <t>Lats2</t> ablation on kidney histopathology after I/R injury. (A) Schematic representation of Lats2-CKO mice established by crossing Lats2flox/flox with γGgt1-Cre+/+ mice. (B) Identification of genotype Lats2flox/flox; γGgt1-Cre+/+ mice by PCR-based genotyping. (C) Lats2-Ctrl and Lats2-CKO mice were subjected to 40 min warm U-IRI. (D) Serum KIM-1 levels of mice were determined by ELISA in sham and U-IRI mice 2 days after U-IRI (sham n = 4, I/R 2d n = 5). (E) Western blotting analysis of LATS2, P-LATS2 <t>(Ser872)</t> in sham and IRI 14d mice and (F) quantified and normalized to GAPDH expression. (G) Representative HE staining of kidney in Lats2-Ctrl and Lats2-CKO mice subjected to U-IRI. Scale bars, 100 µm. (H) Semi-quantification of tubulointerstitial damage (sham n = 4, I/R 14d n = 5). Data expressed as means ± SEM. Two-way ANOVA followed by Tukey’s multiple comparisons post hoc test. * p < 0.05, ** p < 0.01 *** p < 0.001, **** p < 0.0001 defined as significant. ns, not statistically significant.
Polyclonal Af7449, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Gene ontology of 25 selected genes from microarray analyses.

Journal: PLoS ONE

Article Title: The Somatostatin Analogue Octreotide Inhibits Growth of Small Intestine Neuroendocrine Tumour Cells

doi: 10.1371/journal.pone.0048411

Figure Lengend Snippet: Gene ontology of 25 selected genes from microarray analyses.

Article Snippet: Monoclonal mouse anti-human ANXA1 (1∶5000, BD-Transduction Laboratories, Franklin Lakes, NJ, USA), polyclonal rabbit anti-human ARHGAP18 (1∶250, Abgent, San Diego, CA, USA), polyclonal mouse anti-human EMP1 (1∶250, Abnova, Taipei, Taiwan), polyclonal rabbit anti-human GDF15 (1∶250, Atlas Antibodies, Stockholm, Sweden), polyclonal goat anti-human TGFBR2 (1∶250, Santa Cruz Biotechnology, Santa Cruz, CA, USA), polyclonal goat anti-human TNFSF15 (1∶500, R&D Systems, Minneapolis, MN, USA), polyclonal rabbit anti-human SSTR1 (1∶5000, Santa Cruz Biotechnology), polyclonal rabbit anti-human SSTR2 (1∶4000, Thermo Scientific), polyclonal rabbit anti-human SSTR3 and SSTR5 (1∶4000 and 1∶5000, gifts from Frank Leu , polyclonal goat anti-human β-actin and HRP donkey anti-goat (1∶5000, Santa Cruz Biotechnology) and HRP anti-mouse and anti-rabbit (1∶5000, Amersham Biosciences, Buckinghamshire, England) antibodies were used to detect the different proteins.

Techniques: Microarray, Protein Binding, Activity Assay, Transduction, Binding Assay, Membrane, Migration, De-Phosphorylation Assay, Phospho-proteomics

ANXA1, ARHGAP18, EMP1, GDF15, TGFBR2 and TNFSF15 were analysed using total RNA at 1 week (wk), 4 months, 10 months and 16 months (mo) of culture by QRT-PCR. Results were plotted using the 2 −ΔΔCt method with β-actin expression (set to 1) from each individual sample as endogenous reference. Plotted results are means ± SD for triplicate wells. Significance was calculated by Two-Way ANOVA followed by Bonferroni test; comparing with untreated CNDT2.5 cells. *** = p <0.001.

Journal: PLoS ONE

Article Title: The Somatostatin Analogue Octreotide Inhibits Growth of Small Intestine Neuroendocrine Tumour Cells

doi: 10.1371/journal.pone.0048411

Figure Lengend Snippet: ANXA1, ARHGAP18, EMP1, GDF15, TGFBR2 and TNFSF15 were analysed using total RNA at 1 week (wk), 4 months, 10 months and 16 months (mo) of culture by QRT-PCR. Results were plotted using the 2 −ΔΔCt method with β-actin expression (set to 1) from each individual sample as endogenous reference. Plotted results are means ± SD for triplicate wells. Significance was calculated by Two-Way ANOVA followed by Bonferroni test; comparing with untreated CNDT2.5 cells. *** = p <0.001.

Article Snippet: Monoclonal mouse anti-human ANXA1 (1∶5000, BD-Transduction Laboratories, Franklin Lakes, NJ, USA), polyclonal rabbit anti-human ARHGAP18 (1∶250, Abgent, San Diego, CA, USA), polyclonal mouse anti-human EMP1 (1∶250, Abnova, Taipei, Taiwan), polyclonal rabbit anti-human GDF15 (1∶250, Atlas Antibodies, Stockholm, Sweden), polyclonal goat anti-human TGFBR2 (1∶250, Santa Cruz Biotechnology, Santa Cruz, CA, USA), polyclonal goat anti-human TNFSF15 (1∶500, R&D Systems, Minneapolis, MN, USA), polyclonal rabbit anti-human SSTR1 (1∶5000, Santa Cruz Biotechnology), polyclonal rabbit anti-human SSTR2 (1∶4000, Thermo Scientific), polyclonal rabbit anti-human SSTR3 and SSTR5 (1∶4000 and 1∶5000, gifts from Frank Leu , polyclonal goat anti-human β-actin and HRP donkey anti-goat (1∶5000, Santa Cruz Biotechnology) and HRP anti-mouse and anti-rabbit (1∶5000, Amersham Biosciences, Buckinghamshire, England) antibodies were used to detect the different proteins.

Techniques: Quantitative RT-PCR, Expressing

Result of Immunohistochemistry on paraffin embedded SI-NET specimens.

Journal: PLoS ONE

Article Title: The Somatostatin Analogue Octreotide Inhibits Growth of Small Intestine Neuroendocrine Tumour Cells

doi: 10.1371/journal.pone.0048411

Figure Lengend Snippet: Result of Immunohistochemistry on paraffin embedded SI-NET specimens.

Article Snippet: Monoclonal mouse anti-human ANXA1 (1∶5000, BD-Transduction Laboratories, Franklin Lakes, NJ, USA), polyclonal rabbit anti-human ARHGAP18 (1∶250, Abgent, San Diego, CA, USA), polyclonal mouse anti-human EMP1 (1∶250, Abnova, Taipei, Taiwan), polyclonal rabbit anti-human GDF15 (1∶250, Atlas Antibodies, Stockholm, Sweden), polyclonal goat anti-human TGFBR2 (1∶250, Santa Cruz Biotechnology, Santa Cruz, CA, USA), polyclonal goat anti-human TNFSF15 (1∶500, R&D Systems, Minneapolis, MN, USA), polyclonal rabbit anti-human SSTR1 (1∶5000, Santa Cruz Biotechnology), polyclonal rabbit anti-human SSTR2 (1∶4000, Thermo Scientific), polyclonal rabbit anti-human SSTR3 and SSTR5 (1∶4000 and 1∶5000, gifts from Frank Leu , polyclonal goat anti-human β-actin and HRP donkey anti-goat (1∶5000, Santa Cruz Biotechnology) and HRP anti-mouse and anti-rabbit (1∶5000, Amersham Biosciences, Buckinghamshire, England) antibodies were used to detect the different proteins.

Techniques: Immunohistochemistry

Figure 2. The effect of renal proximal tubule-specific Lats2 ablation on kidney histopathology after I/R injury. (A) Schematic representation of Lats2-CKO mice established by crossing Lats2flox/flox with γGgt1-Cre+/+ mice. (B) Identification of genotype Lats2flox/flox; γGgt1-Cre+/+ mice by PCR-based genotyping. (C) Lats2-Ctrl and Lats2-CKO mice were subjected to 40 min warm U-IRI. (D) Serum KIM-1 levels of mice were determined by ELISA in sham and U-IRI mice 2 days after U-IRI (sham n = 4, I/R 2d n = 5). (E) Western blotting analysis of LATS2, P-LATS2 (Ser872) in sham and IRI 14d mice and (F) quantified and normalized to GAPDH expression. (G) Representative HE staining of kidney in Lats2-Ctrl and Lats2-CKO mice subjected to U-IRI. Scale bars, 100 µm. (H) Semi-quantification of tubulointerstitial damage (sham n = 4, I/R 14d n = 5). Data expressed as means ± SEM. Two-way ANOVA followed by Tukey’s multiple comparisons post hoc test. * p < 0.05, ** p < 0.01 *** p < 0.001, **** p < 0.0001 defined as significant. ns, not statistically significant.

Journal: International journal of molecular sciences

Article Title: Proximal Tubular Lats2 Ablation Exacerbates Ischemia/Reperfusion Injury (IRI)-Induced Renal Maladaptive Repair through the Upregulation of P53.

doi: 10.3390/ijms242015258

Figure Lengend Snippet: Figure 2. The effect of renal proximal tubule-specific Lats2 ablation on kidney histopathology after I/R injury. (A) Schematic representation of Lats2-CKO mice established by crossing Lats2flox/flox with γGgt1-Cre+/+ mice. (B) Identification of genotype Lats2flox/flox; γGgt1-Cre+/+ mice by PCR-based genotyping. (C) Lats2-Ctrl and Lats2-CKO mice were subjected to 40 min warm U-IRI. (D) Serum KIM-1 levels of mice were determined by ELISA in sham and U-IRI mice 2 days after U-IRI (sham n = 4, I/R 2d n = 5). (E) Western blotting analysis of LATS2, P-LATS2 (Ser872) in sham and IRI 14d mice and (F) quantified and normalized to GAPDH expression. (G) Representative HE staining of kidney in Lats2-Ctrl and Lats2-CKO mice subjected to U-IRI. Scale bars, 100 µm. (H) Semi-quantification of tubulointerstitial damage (sham n = 4, I/R 14d n = 5). Data expressed as means ± SEM. Two-way ANOVA followed by Tukey’s multiple comparisons post hoc test. * p < 0.05, ** p < 0.01 *** p < 0.001, **** p < 0.0001 defined as significant. ns, not statistically significant.

Article Snippet: After that, the membrane was incubated with the following primary antibodies at 4 ◦C overnight: polyclonal rabbit anti-LATS2 (Cat# 20276-1-AP, Proteintech, Hong Kong, China), polyclonal rabbit antiPhospho-LATS2 (ser872) (Cat# AF7440, Affinity), monoclonal mouse anti-p53 (Cat# sc-126, Santa Cruz, Santa Cruz, CA, USA), monoclonal rabbit anti-p-p53 (Ser15) (Cat# 9284S, CST), monoclonal mouse anti-p21 (Cat# sc-6246, Santa Cruz), monoclonal rabbit anti-Bax (Cat# ab32503, Abcam, Cambridge, UK), polyclonal rabbit anti-cleaved caspase-3 (Cat# AF7022, Affinity), monoclonal rabbit Bcl-xL (Cat# 2764S, CST), monoclonal mouse anti-Bcl-2 (Cat# sc-7382, Santa Cruz), monoclonal rabbit anti-α-SMA (Cat# 19245S, CST), polyclonal rabbit anti-fibronectin (Cat# 15613-1-AP, Proteintech), polyclonal rabbit anti-collagen I (Cat# 14695-1-AP, Proteintech), monoclonal rabbit anti-F4/80 (Cat# 70076S, CST), monoclonal rabbit anti-CD3 (Cat# 99940S, CST), monoclonal rabbit anti-IL-18 (Cat# 57058S, CST), monoclonal rabbit anti-IL-1β (Cat# 12426, CST), polyclonal rabbit anti-phospho-MDM2 (Ser186) (Cat# bs-5471R, Bioss, Woburn, MA, USA), monoclonal mouse anti-β-actin (Cat# sc-47778, Int.

Techniques: Histopathology, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Staining